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(A) Bar plot depicting the relative proportions of immune cell types infiltrated in AAA compared to controls. (B) Differential proportions of immune cell populations between AAA and control groups. The correlation between immune cell types and the expression levels of IL6 (C) , <t>ETS1</t> (D) , TDO2 (E) , and TBX2 (F) in AAA. Kruskal-Walli’s test in (B) , Spearman correlation analysis in (C) , (D) , (E) , and (F) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
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(A) Bar plot depicting the relative proportions of immune cell types infiltrated in AAA compared to controls. (B) Differential proportions of immune cell populations between AAA and control groups. The correlation between immune cell types and the expression levels of IL6 (C) , <t>ETS1</t> (D) , TDO2 (E) , and TBX2 (F) in AAA. Kruskal-Walli’s test in (B) , Spearman correlation analysis in (C) , (D) , (E) , and (F) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
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(A) Bar plot depicting the relative proportions of immune cell types infiltrated in AAA compared to controls. (B) Differential proportions of immune cell populations between AAA and control groups. The correlation between immune cell types and the expression levels of IL6 (C) , <t>ETS1</t> (D) , TDO2 (E) , and TBX2 (F) in AAA. Kruskal-Walli’s test in (B) , Spearman correlation analysis in (C) , (D) , (E) , and (F) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
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(A) Bar plot depicting the relative proportions of immune cell types infiltrated in AAA compared to controls. (B) Differential proportions of immune cell populations between AAA and control groups. The correlation between immune cell types and the expression levels of IL6 (C) , <t>ETS1</t> (D) , TDO2 (E) , and TBX2 (F) in AAA. Kruskal-Walli’s test in (B) , Spearman correlation analysis in (C) , (D) , (E) , and (F) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
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(A) Bar plot depicting the relative proportions of immune cell types infiltrated in AAA compared to controls. (B) Differential proportions of immune cell populations between AAA and control groups. The correlation between immune cell types and the expression levels of IL6 (C) , ETS1 (D) , TDO2 (E) , and TBX2 (F) in AAA. Kruskal-Walli’s test in (B) , Spearman correlation analysis in (C) , (D) , (E) , and (F) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Journal: PLOS One

Article Title: Elucidate senescence-related gene signature and immune infiltration landscape in abdominal aortic aneurysm

doi: 10.1371/journal.pone.0340976

Figure Lengend Snippet: (A) Bar plot depicting the relative proportions of immune cell types infiltrated in AAA compared to controls. (B) Differential proportions of immune cell populations between AAA and control groups. The correlation between immune cell types and the expression levels of IL6 (C) , ETS1 (D) , TDO2 (E) , and TBX2 (F) in AAA. Kruskal-Walli’s test in (B) , Spearman correlation analysis in (C) , (D) , (E) , and (F) . *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Article Snippet: Sections were then deparaffinized in xylene, rehydrated through graded ethanol series, and subjected to immunostaining with anti-ETS1 antibody (12118-1-AP; Proteintech) and anti-TDO2 antibody (15880-1-AP; Proteintech).

Techniques: Control, Expressing

(A) Violin plot showing the distribution of single-cell sequencing data for each AAA sample after quality control. (B) Heatmap displaying the expression of characteristic genes across single-cell subpopulations in AAA tissue. (C) t-SNE plot illustrating the presence of 10 distinct immune cell types within AAA tissue. (D) Dot plot and (E) feather plot presenting the expression patterns of IL6, ETS1, TDO2, and TBX2 across various cell populations.

Journal: PLOS One

Article Title: Elucidate senescence-related gene signature and immune infiltration landscape in abdominal aortic aneurysm

doi: 10.1371/journal.pone.0340976

Figure Lengend Snippet: (A) Violin plot showing the distribution of single-cell sequencing data for each AAA sample after quality control. (B) Heatmap displaying the expression of characteristic genes across single-cell subpopulations in AAA tissue. (C) t-SNE plot illustrating the presence of 10 distinct immune cell types within AAA tissue. (D) Dot plot and (E) feather plot presenting the expression patterns of IL6, ETS1, TDO2, and TBX2 across various cell populations.

Article Snippet: Sections were then deparaffinized in xylene, rehydrated through graded ethanol series, and subjected to immunostaining with anti-ETS1 antibody (12118-1-AP; Proteintech) and anti-TDO2 antibody (15880-1-AP; Proteintech).

Techniques: Sequencing, Control, Expressing

(A) Representative images of the general aorta from sham-operated and AAA groups, with aortic diameter quantified (n = 5). Immunohistochemistry images showing ETS1 (B) and TDO2 (C) staining in sham-operated versus AAA groups. qPCR analysis of IL6, ETS1, TBX2, and TDO2 expression levels in murine aortas (D) and blood (E) 14 days after operation (n = 5). Students’ t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Journal: PLOS One

Article Title: Elucidate senescence-related gene signature and immune infiltration landscape in abdominal aortic aneurysm

doi: 10.1371/journal.pone.0340976

Figure Lengend Snippet: (A) Representative images of the general aorta from sham-operated and AAA groups, with aortic diameter quantified (n = 5). Immunohistochemistry images showing ETS1 (B) and TDO2 (C) staining in sham-operated versus AAA groups. qPCR analysis of IL6, ETS1, TBX2, and TDO2 expression levels in murine aortas (D) and blood (E) 14 days after operation (n = 5). Students’ t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Article Snippet: Sections were then deparaffinized in xylene, rehydrated through graded ethanol series, and subjected to immunostaining with anti-ETS1 antibody (12118-1-AP; Proteintech) and anti-TDO2 antibody (15880-1-AP; Proteintech).

Techniques: Immunohistochemistry, Staining, Expressing